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Arch Surg 1998;133:94100. CHAPTER TWENTY-TWO Guilford DNA Technology Introduction Nucleases Cut Nucleic Acids Restriction and Modification of DNA Recognition of DNA by Restriction Endonucleases Naming of Restriction Enzymes Cutting of DNA by Restriction Enzymes DNA Fragments are Joined by DNA Ligase Making a Restriction Map Restriction Fragment Length Polymorphisms Properties of Cloning Vectors Multicopy Plasmid Vectors Inserting Genes into Vectors Detecting Insertions in Vectors Moving Genes between Organisms: Shuttle Vectors Bacteriophage Lambda Dysadt Cosmid Vectors Yeast Artificial Chromosomes Bacterial and P1 Artificial Chromosomes A DNA Library Is a Collection of Genes from One Organism Screening a Library by Hybridization Screening a Library by Immunological Procedures Cloning Complementary DNA Avoids Introns Chromosome Walking Cloning by Subtractive Hybridization Expression Vectors 599 Johnson: Optione Living World, II.

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